Bismark report for: /gscratch/srlab/sr320/data/cvirg/9M_R1_val_1.fq.gz and /gscratch/srlab/sr320/data/cvirg/9M_R2_val_2.fq.gz (version: v0.22.3) Bismark was run with Bowtie 2 against the bisulfite genome of /gscratch/srlab/sr320/data/Cvirg-genome/ with the specified options: -q --score-min L,0,-0.6 -p 4 --reorder --ignore-quals --no-mixed --no-discordant --dovetail --maxins 500 Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Final Alignment report ====================== Sequence pairs analysed in total: 135520937 Number of paired-end alignments with a unique best hit: 79266140 Mapping efficiency: 58.5% Sequence pairs with no alignments under any condition: 36121166 Sequence pairs did not map uniquely: 20133631 Sequence pairs which were discarded because genomic sequence could not be extracted: 102 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 19858802 ((converted) top strand) GA/CT/CT: 19790665 (complementary to (converted) top strand) GA/CT/GA: 19772967 (complementary to (converted) bottom strand) CT/GA/GA: 19843604 ((converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 3451682585 Total methylated C's in CpG context: 60112195 Total methylated C's in CHG context: 2380787 Total methylated C's in CHH context: 9648034 Total methylated C's in Unknown context: 242012 Total unmethylated C's in CpG context: 394248006 Total unmethylated C's in CHG context: 622580948 Total unmethylated C's in CHH context: 2362712615 Total unmethylated C's in Unknown context: 10829644 C methylated in CpG context: 13.2% C methylated in CHG context: 0.4% C methylated in CHH context: 0.4% C methylated in unknown context (CN or CHN): 2.2% Bismark completed in 0d 13h 49m 59s