Bismark

Bismark Processing Report

/gscratch/srlab/sr320/data/cvirg/9M_R1_val_1.fq.gz and /gscratch/srlab/sr320/data/cvirg/9M_R2_val_2.fq.gz

Data processed at 13:36:57 on 2021-08-12


Alignment Stats

Sequence pairs analysed in total 135520937
Paired-end alignments with a unique best hit 79266140
Pairs without alignments under any condition 36121166
Pairs that did not map uniquely 20133631
Genomic sequence context not extractable (edges of chromosomes) 102

Cytosine Methylation

Total C's analysed 3451682585
Methylated C's in CpG context 60112195
Methylated C's in CHG context 2380787
Methylated C's in CHH context 9648034
Methylated C's in Unknown context 242012
Unmethylated C's in CpG context 394248006
Unmethylated C's in CHG context 622580948
Unmethylated C's in CHH context 2362712615
Unmethylated C's in Unknown context 10829644
Percentage methylation (CpG context) 13.2%
Percentage methylation (CHG context) 0.4%
Percentage methylation (CHH context) 0.4%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 19858802 original top strand
CTOT 19790665 complementary to original top strand
CTOB 19772967 complementary to original bottom strand
OB 19843604 original bottom strand

Deduplication

Alignments analysed 79266038
Unique alignments 73301023
Duplicates removed 5965015
Duplicated alignments were found at 5468634 different positions

Cytosine Methylation after Extraction

Total C's analysed 2060013095
Methylated C's in CpG context 34898355
Methylated C's in CHG context 1383254
Methylated C's in CHH context 5644769
Unmethylated C's in CpG context 232890147
Unmethylated C's in CHG context 367770942
Unmethylated C's in CHH context 1417425628
Percentage methylation (CpG context) 13.0%
Percentage methylation (CHG context) N/A%
Percentage methylation (CHH context) N/A%

M-Bias Plot