Bismark report for: /gscratch/srlab/sr320/data/cvirg/7M_R1_val_1.fq.gz and /gscratch/srlab/sr320/data/cvirg/7M_R2_val_2.fq.gz (version: v0.22.3) Bismark was run with Bowtie 2 against the bisulfite genome of /gscratch/srlab/sr320/data/Cvirg-genome/ with the specified options: -q --score-min L,0,-0.6 -p 4 --reorder --ignore-quals --no-mixed --no-discordant --dovetail --maxins 500 Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Final Alignment report ====================== Sequence pairs analysed in total: 144201637 Number of paired-end alignments with a unique best hit: 82406982 Mapping efficiency: 57.1% Sequence pairs with no alignments under any condition: 40082891 Sequence pairs did not map uniquely: 21711764 Sequence pairs which were discarded because genomic sequence could not be extracted: 135 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 20684348 ((converted) top strand) GA/CT/CT: 20510003 (complementary to (converted) top strand) GA/CT/GA: 20518944 (complementary to (converted) bottom strand) CT/GA/GA: 20693552 ((converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 3542260680 Total methylated C's in CpG context: 64643513 Total methylated C's in CHG context: 2523236 Total methylated C's in CHH context: 10492323 Total methylated C's in Unknown context: 265080 Total unmethylated C's in CpG context: 394013234 Total unmethylated C's in CHG context: 636748264 Total unmethylated C's in CHH context: 2433840110 Total unmethylated C's in Unknown context: 11401161 C methylated in CpG context: 14.1% C methylated in CHG context: 0.4% C methylated in CHH context: 0.4% C methylated in unknown context (CN or CHN): 2.3% Bismark completed in 0d 14h 38m 43s