Bismark

Bismark Processing Report

/gscratch/srlab/sr320/data/cvirg/7M_R1_val_1.fq.gz and /gscratch/srlab/sr320/data/cvirg/7M_R2_val_2.fq.gz

Data processed at 13:36:56 on 2021-08-12


Alignment Stats

Sequence pairs analysed in total 144201637
Paired-end alignments with a unique best hit 82406982
Pairs without alignments under any condition 40082891
Pairs that did not map uniquely 21711764
Genomic sequence context not extractable (edges of chromosomes) 135

Cytosine Methylation

Total C's analysed 3542260680
Methylated C's in CpG context 64643513
Methylated C's in CHG context 2523236
Methylated C's in CHH context 10492323
Methylated C's in Unknown context 265080
Unmethylated C's in CpG context 394013234
Unmethylated C's in CHG context 636748264
Unmethylated C's in CHH context 2433840110
Unmethylated C's in Unknown context 11401161
Percentage methylation (CpG context) 14.1%
Percentage methylation (CHG context) 0.4%
Percentage methylation (CHH context) 0.4%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 20684348 original top strand
CTOT 20510003 complementary to original top strand
CTOB 20518944 complementary to original bottom strand
OB 20693552 original bottom strand

Deduplication

Alignments analysed 82406847
Unique alignments 75968903
Duplicates removed 6437944
Duplicated alignments were found at 5875780 different positions

Cytosine Methylation after Extraction

Total C's analysed 2148117321
Methylated C's in CpG context 38122812
Methylated C's in CHG context 1485354
Methylated C's in CHH context 6163716
Unmethylated C's in CpG context 236284094
Unmethylated C's in CHG context 381726093
Unmethylated C's in CHH context 1484335252
Percentage methylation (CpG context) 13.9%
Percentage methylation (CHG context) N/A%
Percentage methylation (CHH context) N/A%

M-Bias Plot