Bismark

Bismark Processing Report

/gscratch/srlab/sr320/data/cvirg/77F_R1_val_1.fq.gz and /gscratch/srlab/sr320/data/cvirg/77F_R2_val_2.fq.gz

Data processed at 13:36:56 on 2021-08-12


Alignment Stats

Sequence pairs analysed in total 183300828
Paired-end alignments with a unique best hit 105710132
Pairs without alignments under any condition 49753928
Pairs that did not map uniquely 27836768
Genomic sequence context not extractable (edges of chromosomes) 1428

Cytosine Methylation

Total C's analysed 4419317856
Methylated C's in CpG context 66262743
Methylated C's in CHG context 3100353
Methylated C's in CHH context 12655064
Methylated C's in Unknown context 288225
Unmethylated C's in CpG context 507907192
Unmethylated C's in CHG context 789148399
Unmethylated C's in CHH context 3040244105
Unmethylated C's in Unknown context 13811814
Percentage methylation (CpG context) 11.5%
Percentage methylation (CHG context) 0.4%
Percentage methylation (CHH context) 0.4%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 26698380 original top strand
CTOT 26560878 complementary to original top strand
CTOB 26145268 complementary to original bottom strand
OB 26304178 original bottom strand

Deduplication

Alignments analysed 105708704
Unique alignments 95462715
Duplicates removed 10245989
Duplicated alignments were found at 8558972 different positions

Cytosine Methylation after Extraction

Total C's analysed 2499600963
Methylated C's in CpG context 36760446
Methylated C's in CHG context 1702958
Methylated C's in CHH context 7092183
Unmethylated C's in CpG context 283258341
Unmethylated C's in CHG context 441615989
Unmethylated C's in CHH context 1729171046
Percentage methylation (CpG context) 11.5%
Percentage methylation (CHG context) N/A%
Percentage methylation (CHH context) N/A%

M-Bias Plot