Bismark

Bismark Processing Report

/gscratch/srlab/sr320/data/cvirg/76F_R1_val_1.fq.gz and /gscratch/srlab/sr320/data/cvirg/76F_R2_val_2.fq.gz

Data processed at 13:36:56 on 2021-08-12


Alignment Stats

Sequence pairs analysed in total 155271055
Paired-end alignments with a unique best hit 91161529
Pairs without alignments under any condition 41173079
Pairs that did not map uniquely 22936447
Genomic sequence context not extractable (edges of chromosomes) 1230

Cytosine Methylation

Total C's analysed 3862098313
Methylated C's in CpG context 60581431
Methylated C's in CHG context 2720254
Methylated C's in CHH context 12090716
Methylated C's in Unknown context 271187
Unmethylated C's in CpG context 441252446
Unmethylated C's in CHG context 691448235
Unmethylated C's in CHH context 2654005231
Unmethylated C's in Unknown context 12231242
Percentage methylation (CpG context) 12.1%
Percentage methylation (CHG context) 0.4%
Percentage methylation (CHH context) 0.5%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 23055743 original top strand
CTOT 22910539 complementary to original top strand
CTOB 22526464 complementary to original bottom strand
OB 22667553 original bottom strand

Deduplication

Alignments analysed 91160299
Unique alignments 82560622
Duplicates removed 8599677
Duplicated alignments were found at 7152453 different positions

Cytosine Methylation after Extraction

Total C's analysed 2243661587
Methylated C's in CpG context 34487480
Methylated C's in CHG context 1538367
Methylated C's in CHH context 6745430
Unmethylated C's in CpG context 252523035
Unmethylated C's in CHG context 397100054
Unmethylated C's in CHH context 1551267221
Percentage methylation (CpG context) 12.0%
Percentage methylation (CHG context) N/A%
Percentage methylation (CHH context) N/A%

M-Bias Plot