Bismark report for: /gscratch/srlab/sr320/data/cvirg/6M_R1_val_1.fq.gz and /gscratch/srlab/sr320/data/cvirg/6M_R2_val_2.fq.gz (version: v0.22.3) Bismark was run with Bowtie 2 against the bisulfite genome of /gscratch/srlab/sr320/data/Cvirg-genome/ with the specified options: -q --score-min L,0,-0.6 -p 4 --reorder --ignore-quals --no-mixed --no-discordant --dovetail --maxins 500 Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Final Alignment report ====================== Sequence pairs analysed in total: 156817982 Number of paired-end alignments with a unique best hit: 87759040 Mapping efficiency: 56.0% Sequence pairs with no alignments under any condition: 43018405 Sequence pairs did not map uniquely: 26040537 Sequence pairs which were discarded because genomic sequence could not be extracted: 109 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 21781502 ((converted) top strand) GA/CT/CT: 22074312 (complementary to (converted) top strand) GA/CT/GA: 22094808 (complementary to (converted) bottom strand) CT/GA/GA: 21808309 ((converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 3628530531 Total methylated C's in CpG context: 69347460 Total methylated C's in CHG context: 3029600 Total methylated C's in CHH context: 14405917 Total methylated C's in Unknown context: 368349 Total unmethylated C's in CpG context: 443733583 Total unmethylated C's in CHG context: 698908031 Total unmethylated C's in CHH context: 2399105940 Total unmethylated C's in Unknown context: 11131292 C methylated in CpG context: 13.5% C methylated in CHG context: 0.4% C methylated in CHH context: 0.6% C methylated in unknown context (CN or CHN): 3.2% Bismark completed in 0d 14h 54m 19s