Bismark

Bismark Processing Report

/gscratch/srlab/sr320/data/cvirg/6M_R1_val_1.fq.gz and /gscratch/srlab/sr320/data/cvirg/6M_R2_val_2.fq.gz

Data processed at 13:36:56 on 2021-08-12


Alignment Stats

Sequence pairs analysed in total 156817982
Paired-end alignments with a unique best hit 87759040
Pairs without alignments under any condition 43018405
Pairs that did not map uniquely 26040537
Genomic sequence context not extractable (edges of chromosomes) 109

Cytosine Methylation

Total C's analysed 3628530531
Methylated C's in CpG context 69347460
Methylated C's in CHG context 3029600
Methylated C's in CHH context 14405917
Methylated C's in Unknown context 368349
Unmethylated C's in CpG context 443733583
Unmethylated C's in CHG context 698908031
Unmethylated C's in CHH context 2399105940
Unmethylated C's in Unknown context 11131292
Percentage methylation (CpG context) 13.5%
Percentage methylation (CHG context) 0.4%
Percentage methylation (CHH context) 0.6%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 21781502 original top strand
CTOT 22074312 complementary to original top strand
CTOB 22094808 complementary to original bottom strand
OB 21808309 original bottom strand

Deduplication

Alignments analysed 87758931
Unique alignments 80219444
Duplicates removed 7539487
Duplicated alignments were found at 6731573 different positions

Cytosine Methylation after Extraction

Total C's analysed 1995315281
Methylated C's in CpG context 36932556
Methylated C's in CHG context 1621071
Methylated C's in CHH context 7648031
Unmethylated C's in CpG context 241004510
Unmethylated C's in CHG context 379429880
Unmethylated C's in CHH context 1328679233
Percentage methylation (CpG context) 13.3%
Percentage methylation (CHG context) N/A%
Percentage methylation (CHH context) N/A%

M-Bias Plot