Bismark

Bismark Processing Report

/gscratch/srlab/sr320/data/cvirg/64M_R1_val_1.fq.gz and /gscratch/srlab/sr320/data/cvirg/64M_R2_val_2.fq.gz

Data processed at 13:36:56 on 2021-08-12


Alignment Stats

Sequence pairs analysed in total 168612201
Paired-end alignments with a unique best hit 100387649
Pairs without alignments under any condition 42325464
Pairs that did not map uniquely 25899088
Genomic sequence context not extractable (edges of chromosomes) 183

Cytosine Methylation

Total C's analysed 4255006376
Methylated C's in CpG context 74469637
Methylated C's in CHG context 3141876
Methylated C's in CHH context 12821712
Methylated C's in Unknown context 300871
Unmethylated C's in CpG context 479743410
Unmethylated C's in CHG context 760913719
Unmethylated C's in CHH context 2923916022
Unmethylated C's in Unknown context 13388464
Percentage methylation (CpG context) 13.4%
Percentage methylation (CHG context) 0.4%
Percentage methylation (CHH context) 0.4%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 25135096 original top strand
CTOT 25029751 complementary to original top strand
CTOB 25053867 complementary to original bottom strand
OB 25168752 original bottom strand

Deduplication

Alignments analysed 100387466
Unique alignments 91896803
Duplicates removed 8490663
Duplicated alignments were found at 7687406 different positions

Cytosine Methylation after Extraction

Total C's analysed 2479740246
Methylated C's in CpG context 42206303
Methylated C's in CHG context 1779052
Methylated C's in CHH context 7273814
Unmethylated C's in CpG context 276397190
Unmethylated C's in CHG context 438750654
Unmethylated C's in CHH context 1713333233
Percentage methylation (CpG context) 13.2%
Percentage methylation (CHG context) N/A%
Percentage methylation (CHH context) N/A%

M-Bias Plot