Bismark report for: /gscratch/srlab/sr320/data/cvirg/59M_R1_val_1.fq.gz and /gscratch/srlab/sr320/data/cvirg/59M_R2_val_2.fq.gz (version: v0.22.3) Bismark was run with Bowtie 2 against the bisulfite genome of /gscratch/srlab/sr320/data/Cvirg-genome/ with the specified options: -q --score-min L,0,-0.6 -p 4 --reorder --ignore-quals --no-mixed --no-discordant --dovetail --maxins 500 Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Final Alignment report ====================== Sequence pairs analysed in total: 140047179 Number of paired-end alignments with a unique best hit: 80039790 Mapping efficiency: 57.2% Sequence pairs with no alignments under any condition: 34620424 Sequence pairs did not map uniquely: 25386965 Sequence pairs which were discarded because genomic sequence could not be extracted: 110 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 20029357 ((converted) top strand) GA/CT/CT: 19976209 (complementary to (converted) top strand) GA/CT/GA: 19987409 (complementary to (converted) bottom strand) CT/GA/GA: 20046705 ((converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 3113845939 Total methylated C's in CpG context: 60813384 Total methylated C's in CHG context: 2325913 Total methylated C's in CHH context: 9784189 Total methylated C's in Unknown context: 247025 Total unmethylated C's in CpG context: 363464595 Total unmethylated C's in CHG context: 577415501 Total unmethylated C's in CHH context: 2100042357 Total unmethylated C's in Unknown context: 9330745 C methylated in CpG context: 14.3% C methylated in CHG context: 0.4% C methylated in CHH context: 0.5% C methylated in unknown context (CN or CHN): 2.6% Bismark completed in 0d 13h 9m 30s