Bismark

Bismark Processing Report

/gscratch/srlab/sr320/data/cvirg/59M_R1_val_1.fq.gz and /gscratch/srlab/sr320/data/cvirg/59M_R2_val_2.fq.gz

Data processed at 13:36:56 on 2021-08-12


Alignment Stats

Sequence pairs analysed in total 140047179
Paired-end alignments with a unique best hit 80039790
Pairs without alignments under any condition 34620424
Pairs that did not map uniquely 25386965
Genomic sequence context not extractable (edges of chromosomes) 110

Cytosine Methylation

Total C's analysed 3113845939
Methylated C's in CpG context 60813384
Methylated C's in CHG context 2325913
Methylated C's in CHH context 9784189
Methylated C's in Unknown context 247025
Unmethylated C's in CpG context 363464595
Unmethylated C's in CHG context 577415501
Unmethylated C's in CHH context 2100042357
Unmethylated C's in Unknown context 9330745
Percentage methylation (CpG context) 14.3%
Percentage methylation (CHG context) 0.4%
Percentage methylation (CHH context) 0.5%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 20029357 original top strand
CTOT 19976209 complementary to original top strand
CTOB 19987409 complementary to original bottom strand
OB 20046705 original bottom strand

Deduplication

Alignments analysed 80039680
Unique alignments 73233985
Duplicates removed 6805695
Duplicated alignments were found at 6156538 different positions

Cytosine Methylation after Extraction

Total C's analysed 1689141290
Methylated C's in CpG context 31866013
Methylated C's in CHG context 1219594
Methylated C's in CHH context 5167236
Unmethylated C's in CpG context 194609831
Unmethylated C's in CHG context 309497248
Unmethylated C's in CHH context 1146781368
Percentage methylation (CpG context) 14.1%
Percentage methylation (CHG context) N/A%
Percentage methylation (CHH context) N/A%

M-Bias Plot