Bismark

Bismark Processing Report

/gscratch/srlab/sr320/data/cvirg/54F_R1_val_1.fq.gz and /gscratch/srlab/sr320/data/cvirg/54F_R2_val_2.fq.gz

Data processed at 13:36:55 on 2021-08-12


Alignment Stats

Sequence pairs analysed in total 198527033
Paired-end alignments with a unique best hit 105571623
Pairs without alignments under any condition 68325477
Pairs that did not map uniquely 24629933
Genomic sequence context not extractable (edges of chromosomes) 718

Cytosine Methylation

Total C's analysed 4489602415
Methylated C's in CpG context 67942623
Methylated C's in CHG context 3168438
Methylated C's in CHH context 12958698
Methylated C's in Unknown context 291793
Unmethylated C's in CpG context 499425422
Unmethylated C's in CHG context 787316597
Unmethylated C's in CHH context 3118790637
Unmethylated C's in Unknown context 14530699
Percentage methylation (CpG context) 12.0%
Percentage methylation (CHG context) 0.4%
Percentage methylation (CHH context) 0.4%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 26510441 original top strand
CTOT 26382330 complementary to original top strand
CTOB 26269629 complementary to original bottom strand
OB 26408505 original bottom strand

Deduplication

Alignments analysed 105570905
Unique alignments 94455697
Duplicates removed 11115208
Duplicated alignments were found at 9824762 different positions

Cytosine Methylation after Extraction

Total C's analysed 2648996482
Methylated C's in CpG context 39062781
Methylated C's in CHG context 1817935
Methylated C's in CHH context 7450923
Unmethylated C's in CpG context 291237285
Unmethylated C's in CHG context 459726104
Unmethylated C's in CHH context 1849701454
Percentage methylation (CpG context) 11.8%
Percentage methylation (CHG context) N/A%
Percentage methylation (CHH context) N/A%

M-Bias Plot