Bismark report for: /gscratch/srlab/sr320/data/cvirg/53F_R1_val_1.fq.gz and /gscratch/srlab/sr320/data/cvirg/53F_R2_val_2.fq.gz (version: v0.22.3) Bismark was run with Bowtie 2 against the bisulfite genome of /gscratch/srlab/sr320/data/Cvirg-genome/ with the specified options: -q --score-min L,0,-0.6 -p 4 --reorder --ignore-quals --no-mixed --no-discordant --dovetail --maxins 500 Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Final Alignment report ====================== Sequence pairs analysed in total: 171116745 Number of paired-end alignments with a unique best hit: 99736023 Mapping efficiency: 58.3% Sequence pairs with no alignments under any condition: 47365652 Sequence pairs did not map uniquely: 24015070 Sequence pairs which were discarded because genomic sequence could not be extracted: 1297 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 25110290 ((converted) top strand) GA/CT/CT: 24871801 (complementary to (converted) top strand) GA/CT/GA: 24748499 (complementary to (converted) bottom strand) CT/GA/GA: 25004136 ((converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 4213397728 Total methylated C's in CpG context: 63588936 Total methylated C's in CHG context: 2999855 Total methylated C's in CHH context: 12088410 Total methylated C's in Unknown context: 265748 Total unmethylated C's in CpG context: 468154619 Total unmethylated C's in CHG context: 732034413 Total unmethylated C's in CHH context: 2934531495 Total unmethylated C's in Unknown context: 13679528 C methylated in CpG context: 12.0% C methylated in CHG context: 0.4% C methylated in CHH context: 0.4% C methylated in unknown context (CN or CHN): 1.9% Bismark completed in 0d 17h 24m 33s