Bismark report for: /gscratch/srlab/sr320/data/cvirg/52F_R1_val_1.fq.gz and /gscratch/srlab/sr320/data/cvirg/52F_R2_val_2.fq.gz (version: v0.22.3) Bismark was run with Bowtie 2 against the bisulfite genome of /gscratch/srlab/sr320/data/Cvirg-genome/ with the specified options: -q --score-min L,0,-0.6 -p 4 --reorder --ignore-quals --no-mixed --no-discordant --dovetail --maxins 500 Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Final Alignment report ====================== Sequence pairs analysed in total: 174210505 Number of paired-end alignments with a unique best hit: 98981177 Mapping efficiency: 56.8% Sequence pairs with no alignments under any condition: 52259129 Sequence pairs did not map uniquely: 22970199 Sequence pairs which were discarded because genomic sequence could not be extracted: 370 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 25019409 ((converted) top strand) GA/CT/CT: 24511357 (complementary to (converted) top strand) GA/CT/GA: 24471994 (complementary to (converted) bottom strand) CT/GA/GA: 24978047 ((converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 4207778543 Total methylated C's in CpG context: 62667593 Total methylated C's in CHG context: 2949453 Total methylated C's in CHH context: 12131312 Total methylated C's in Unknown context: 266061 Total unmethylated C's in CpG context: 465500611 Total unmethylated C's in CHG context: 728104532 Total unmethylated C's in CHH context: 2936425042 Total unmethylated C's in Unknown context: 13731359 C methylated in CpG context: 11.9% C methylated in CHG context: 0.4% C methylated in CHH context: 0.4% C methylated in unknown context (CN or CHN): 1.9% Bismark completed in 0d 17h 28m 11s