Bismark

Bismark Processing Report

/gscratch/srlab/sr320/data/cvirg/52F_R1_val_1.fq.gz and /gscratch/srlab/sr320/data/cvirg/52F_R2_val_2.fq.gz

Data processed at 13:36:55 on 2021-08-12


Alignment Stats

Sequence pairs analysed in total 174210505
Paired-end alignments with a unique best hit 98981177
Pairs without alignments under any condition 52259129
Pairs that did not map uniquely 22970199
Genomic sequence context not extractable (edges of chromosomes) 370

Cytosine Methylation

Total C's analysed 4207778543
Methylated C's in CpG context 62667593
Methylated C's in CHG context 2949453
Methylated C's in CHH context 12131312
Methylated C's in Unknown context 266061
Unmethylated C's in CpG context 465500611
Unmethylated C's in CHG context 728104532
Unmethylated C's in CHH context 2936425042
Unmethylated C's in Unknown context 13731359
Percentage methylation (CpG context) 11.9%
Percentage methylation (CHG context) 0.4%
Percentage methylation (CHH context) 0.4%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 25019409 original top strand
CTOT 24511357 complementary to original top strand
CTOB 24471994 complementary to original bottom strand
OB 24978047 original bottom strand

Deduplication

Alignments analysed 98980807
Unique alignments 90021414
Duplicates removed 8959393
Duplicated alignments were found at 8100580 different positions

Cytosine Methylation after Extraction

Total C's analysed 2568366686
Methylated C's in CpG context 37267533
Methylated C's in CHG context 1750935
Methylated C's in CHH context 7241846
Unmethylated C's in CpG context 280880651
Unmethylated C's in CHG context 440074373
Unmethylated C's in CHH context 1801151348
Percentage methylation (CpG context) 11.7%
Percentage methylation (CHG context) N/A%
Percentage methylation (CHH context) N/A%

M-Bias Plot