Bismark report for: /gscratch/srlab/sr320/data/cvirg/50F_R1_val_1.fq.gz and /gscratch/srlab/sr320/data/cvirg/50F_R2_val_2.fq.gz (version: v0.22.3) Bismark was run with Bowtie 2 against the bisulfite genome of /gscratch/srlab/sr320/data/Cvirg-genome/ with the specified options: -q --score-min L,0,-0.6 -p 4 --reorder --ignore-quals --no-mixed --no-discordant --dovetail --maxins 500 Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Final Alignment report ====================== Sequence pairs analysed in total: 147179212 Number of paired-end alignments with a unique best hit: 83381318 Mapping efficiency: 56.7% Sequence pairs with no alignments under any condition: 45245169 Sequence pairs did not map uniquely: 18552725 Sequence pairs which were discarded because genomic sequence could not be extracted: 502 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 20941531 ((converted) top strand) GA/CT/CT: 20857716 (complementary to (converted) top strand) GA/CT/GA: 20742134 (complementary to (converted) bottom strand) CT/GA/GA: 20839435 ((converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 3568167604 Total methylated C's in CpG context: 54317296 Total methylated C's in CHG context: 2629468 Total methylated C's in CHH context: 11006199 Total methylated C's in Unknown context: 248689 Total unmethylated C's in CpG context: 395052270 Total unmethylated C's in CHG context: 624289769 Total unmethylated C's in CHH context: 2480872602 Total unmethylated C's in Unknown context: 11791014 C methylated in CpG context: 12.1% C methylated in CHG context: 0.4% C methylated in CHH context: 0.4% C methylated in unknown context (CN or CHN): 2.1% Bismark completed in 0d 14h 52m 10s