Bismark report for: /gscratch/srlab/sr320/data/cvirg/48M_R1_val_1.fq.gz and /gscratch/srlab/sr320/data/cvirg/48M_R2_val_2.fq.gz (version: v0.22.3) Bismark was run with Bowtie 2 against the bisulfite genome of /gscratch/srlab/sr320/data/Cvirg-genome/ with the specified options: -q --score-min L,0,-0.6 -p 4 --reorder --ignore-quals --no-mixed --no-discordant --dovetail --maxins 500 Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Final Alignment report ====================== Sequence pairs analysed in total: 202125624 Number of paired-end alignments with a unique best hit: 115530829 Mapping efficiency: 57.2% Sequence pairs with no alignments under any condition: 50836754 Sequence pairs did not map uniquely: 35758041 Sequence pairs which were discarded because genomic sequence could not be extracted: 132 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 28924082 ((converted) top strand) GA/CT/CT: 28849225 (complementary to (converted) top strand) GA/CT/GA: 28842857 (complementary to (converted) bottom strand) CT/GA/GA: 28914533 ((converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 4790942878 Total methylated C's in CpG context: 89079471 Total methylated C's in CHG context: 3760708 Total methylated C's in CHH context: 18396502 Total methylated C's in Unknown context: 414558 Total unmethylated C's in CpG context: 571435071 Total unmethylated C's in CHG context: 921433272 Total unmethylated C's in CHH context: 3186837854 Total unmethylated C's in Unknown context: 14527659 C methylated in CpG context: 13.5% C methylated in CHG context: 0.4% C methylated in CHH context: 0.6% C methylated in unknown context (CN or CHN): 2.8% Bismark completed in 0d 19h 32m 47s