Bismark

Bismark Processing Report

/gscratch/srlab/sr320/data/cvirg/48M_R1_val_1.fq.gz and /gscratch/srlab/sr320/data/cvirg/48M_R2_val_2.fq.gz

Data processed at 13:36:55 on 2021-08-12


Alignment Stats

Sequence pairs analysed in total 202125624
Paired-end alignments with a unique best hit 115530829
Pairs without alignments under any condition 50836754
Pairs that did not map uniquely 35758041
Genomic sequence context not extractable (edges of chromosomes) 132
57.2%25.2%17.7%0.0000653%
Unique AlignmentsNo AlignmentMultiple AlignmentsNo Genomic Sequence

Cytosine Methylation

Total C's analysed 4790942878
Methylated C's in CpG context 89079471
Methylated C's in CHG context 3760708
Methylated C's in CHH context 18396502
Methylated C's in Unknown context 414558
Unmethylated C's in CpG context 571435071
Unmethylated C's in CHG context 921433272
Unmethylated C's in CHH context 3186837854
Unmethylated C's in Unknown context 14527659
Percentage methylation (CpG context) 13.5%
Percentage methylation (CHG context) 0.4%
Percentage methylation (CHH context) 0.6%
Methylated C's in Unknown context N/A%
CpG contextCHG contextCHH context020406080100
% Methylation

Alignment to Individual Bisulfite Strands

OT 28924082 original top strand
CTOT 28849225 complementary to original top strand
CTOB 28842857 complementary to original bottom strand
OB 28914533 original bottom strand
OTCTOTCTOBOB05M10M15M20M25M30M
Number of Alignments

Deduplication

Alignments analysed 115530697
Unique alignments 103543900
Duplicates removed 11986797
Duplicated alignments were found at 10417713 different positions
89.6%10.4%
Unique AlignmentsDuplicate Alignments

Cytosine Methylation after Extraction

Total C's analysed 2570349030
Methylated C's in CpG context 46430852
Methylated C's in CHG context 1955189
Methylated C's in CHH context 9333350
Unmethylated C's in CpG context 302893637
Unmethylated C's in CHG context 487842570
Unmethylated C's in CHH context 1721893432
Percentage methylation (CpG context) 13.3%
Percentage methylation (CHG context) N/A%
Percentage methylation (CHH context) N/A%
CpG contextCHH contextCHG context020406080100
% Methylation

M-Bias Plot

204060801001200204060801002M4M6M8M10M12M
CpG methylationCHG methylationCHH methylationCpG total callsCHG total callsCHH total callsRead 1Position in Read [bp]% Methylation# Methylation Calls

2040608010012002040608010001M2M3M4M5M
CpG methylationCHG methylationCHH methylationCpG total callsCHG total callsCHH total callsRead 2Position in Read [bp]% Methylation# Methylation Calls