Bismark report for: /gscratch/srlab/sr320/data/cvirg/3F_R1_val_1.fq.gz and /gscratch/srlab/sr320/data/cvirg/3F_R2_val_2.fq.gz (version: v0.22.3) Bismark was run with Bowtie 2 against the bisulfite genome of /gscratch/srlab/sr320/data/Cvirg-genome/ with the specified options: -q --score-min L,0,-0.6 -p 4 --reorder --ignore-quals --no-mixed --no-discordant --dovetail --maxins 500 Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Final Alignment report ====================== Sequence pairs analysed in total: 175332621 Number of paired-end alignments with a unique best hit: 94818304 Mapping efficiency: 54.1% Sequence pairs with no alignments under any condition: 56280666 Sequence pairs did not map uniquely: 24233651 Sequence pairs which were discarded because genomic sequence could not be extracted: 779 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 23859022 ((converted) top strand) GA/CT/CT: 23742044 (complementary to (converted) top strand) GA/CT/GA: 23539465 (complementary to (converted) bottom strand) CT/GA/GA: 23676994 ((converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 3881124484 Total methylated C's in CpG context: 59562205 Total methylated C's in CHG context: 2845441 Total methylated C's in CHH context: 12466440 Total methylated C's in Unknown context: 289535 Total unmethylated C's in CpG context: 453919526 Total unmethylated C's in CHG context: 696033895 Total unmethylated C's in CHH context: 2656296977 Total unmethylated C's in Unknown context: 11856812 C methylated in CpG context: 11.6% C methylated in CHG context: 0.4% C methylated in CHH context: 0.5% C methylated in unknown context (CN or CHN): 2.4% Bismark completed in 0d 16h 34m 5s