Bismark report for: /gscratch/srlab/sr320/data/cvirg/39F_R1_val_1.fq.gz and /gscratch/srlab/sr320/data/cvirg/39F_R2_val_2.fq.gz (version: v0.22.3) Bismark was run with Bowtie 2 against the bisulfite genome of /gscratch/srlab/sr320/data/Cvirg-genome/ with the specified options: -q --score-min L,0,-0.6 -p 4 --reorder --ignore-quals --no-mixed --no-discordant --dovetail --maxins 500 Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Final Alignment report ====================== Sequence pairs analysed in total: 156641108 Number of paired-end alignments with a unique best hit: 88891085 Mapping efficiency: 56.7% Sequence pairs with no alignments under any condition: 44785775 Sequence pairs did not map uniquely: 22964248 Sequence pairs which were discarded because genomic sequence could not be extracted: 1498 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 22395048 ((converted) top strand) GA/CT/CT: 22304123 (complementary to (converted) top strand) GA/CT/GA: 22052360 (complementary to (converted) bottom strand) CT/GA/GA: 22138056 ((converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 3682137464 Total methylated C's in CpG context: 57008069 Total methylated C's in CHG context: 2722172 Total methylated C's in CHH context: 11308743 Total methylated C's in Unknown context: 285118 Total unmethylated C's in CpG context: 420738828 Total unmethylated C's in CHG context: 650131464 Total unmethylated C's in CHH context: 2540228188 Total unmethylated C's in Unknown context: 11828259 C methylated in CpG context: 11.9% C methylated in CHG context: 0.4% C methylated in CHH context: 0.4% C methylated in unknown context (CN or CHN): 2.4% Bismark completed in 0d 15h 21m 45s