Bismark

Bismark Processing Report

/gscratch/srlab/sr320/data/cvirg/39F_R1_val_1.fq.gz and /gscratch/srlab/sr320/data/cvirg/39F_R2_val_2.fq.gz

Data processed at 13:36:54 on 2021-08-12


Alignment Stats

Sequence pairs analysed in total 156641108
Paired-end alignments with a unique best hit 88891085
Pairs without alignments under any condition 44785775
Pairs that did not map uniquely 22964248
Genomic sequence context not extractable (edges of chromosomes) 1498

Cytosine Methylation

Total C's analysed 3682137464
Methylated C's in CpG context 57008069
Methylated C's in CHG context 2722172
Methylated C's in CHH context 11308743
Methylated C's in Unknown context 285118
Unmethylated C's in CpG context 420738828
Unmethylated C's in CHG context 650131464
Unmethylated C's in CHH context 2540228188
Unmethylated C's in Unknown context 11828259
Percentage methylation (CpG context) 11.9%
Percentage methylation (CHG context) 0.4%
Percentage methylation (CHH context) 0.4%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 22395048 original top strand
CTOT 22304123 complementary to original top strand
CTOB 22052360 complementary to original bottom strand
OB 22138056 original bottom strand

Deduplication

Alignments analysed 88889587
Unique alignments 80072194
Duplicates removed 8817393
Duplicated alignments were found at 7355701 different positions

Cytosine Methylation after Extraction

Total C's analysed 2074276116
Methylated C's in CpG context 31536948
Methylated C's in CHG context 1496914
Methylated C's in CHH context 6317241
Unmethylated C's in CpG context 233554310
Unmethylated C's in CHG context 362416138
Unmethylated C's in CHH context 1438954565
Percentage methylation (CpG context) 11.9%
Percentage methylation (CHG context) N/A%
Percentage methylation (CHH context) N/A%

M-Bias Plot