Bismark

Bismark Processing Report

/gscratch/srlab/sr320/data/cvirg/36F_R1_val_1.fq.gz and /gscratch/srlab/sr320/data/cvirg/36F_R2_val_2.fq.gz

Data processed at 13:36:54 on 2021-08-12


Alignment Stats

Sequence pairs analysed in total 163408505
Paired-end alignments with a unique best hit 93893663
Pairs without alignments under any condition 45977126
Pairs that did not map uniquely 23537716
Genomic sequence context not extractable (edges of chromosomes) 1070

Cytosine Methylation

Total C's analysed 3956409395
Methylated C's in CpG context 59938704
Methylated C's in CHG context 3056288
Methylated C's in CHH context 12809039
Methylated C's in Unknown context 275272
Unmethylated C's in CpG context 448891959
Unmethylated C's in CHG context 696190459
Unmethylated C's in CHH context 2735522946
Unmethylated C's in Unknown context 12801196
Percentage methylation (CpG context) 11.8%
Percentage methylation (CHG context) 0.4%
Percentage methylation (CHH context) 0.5%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 23677898 original top strand
CTOT 23472350 complementary to original top strand
CTOB 23271930 complementary to original bottom strand
OB 23470415 original bottom strand

Deduplication

Alignments analysed 93892593
Unique alignments 85207013
Duplicates removed 8685580
Duplicated alignments were found at 7444146 different positions

Cytosine Methylation after Extraction

Total C's analysed 2328124966
Methylated C's in CpG context 34436105
Methylated C's in CHG context 1741368
Methylated C's in CHH context 7322382
Unmethylated C's in CpG context 260760867
Unmethylated C's in CHG context 405189661
Unmethylated C's in CHH context 1618674583
Percentage methylation (CpG context) 11.7%
Percentage methylation (CHG context) N/A%
Percentage methylation (CHH context) N/A%

M-Bias Plot