Bismark report for: /gscratch/srlab/sr320/data/cvirg/35F_R1_val_1.fq.gz and /gscratch/srlab/sr320/data/cvirg/35F_R2_val_2.fq.gz (version: v0.22.3) Bismark was run with Bowtie 2 against the bisulfite genome of /gscratch/srlab/sr320/data/Cvirg-genome/ with the specified options: -q --score-min L,0,-0.6 -p 4 --reorder --ignore-quals --no-mixed --no-discordant --dovetail --maxins 500 Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Final Alignment report ====================== Sequence pairs analysed in total: 159213229 Number of paired-end alignments with a unique best hit: 91807146 Mapping efficiency: 57.7% Sequence pairs with no alignments under any condition: 45218941 Sequence pairs did not map uniquely: 22187142 Sequence pairs which were discarded because genomic sequence could not be extracted: 1560 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 22935222 ((converted) top strand) GA/CT/CT: 23179245 (complementary to (converted) top strand) GA/CT/GA: 22958193 (complementary to (converted) bottom strand) CT/GA/GA: 22732926 ((converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 3827582704 Total methylated C's in CpG context: 59205639 Total methylated C's in CHG context: 2858946 Total methylated C's in CHH context: 11415266 Total methylated C's in Unknown context: 249940 Total unmethylated C's in CpG context: 430606533 Total unmethylated C's in CHG context: 670791467 Total unmethylated C's in CHH context: 2652704853 Total unmethylated C's in Unknown context: 12145715 C methylated in CpG context: 12.1% C methylated in CHG context: 0.4% C methylated in CHH context: 0.4% C methylated in unknown context (CN or CHN): 2.0% Bismark completed in 0d 15h 56m 42s