Bismark

Bismark Processing Report

/gscratch/srlab/sr320/data/cvirg/35F_R1_val_1.fq.gz and /gscratch/srlab/sr320/data/cvirg/35F_R2_val_2.fq.gz

Data processed at 13:36:54 on 2021-08-12


Alignment Stats

Sequence pairs analysed in total 159213229
Paired-end alignments with a unique best hit 91807146
Pairs without alignments under any condition 45218941
Pairs that did not map uniquely 22187142
Genomic sequence context not extractable (edges of chromosomes) 1560

Cytosine Methylation

Total C's analysed 3827582704
Methylated C's in CpG context 59205639
Methylated C's in CHG context 2858946
Methylated C's in CHH context 11415266
Methylated C's in Unknown context 249940
Unmethylated C's in CpG context 430606533
Unmethylated C's in CHG context 670791467
Unmethylated C's in CHH context 2652704853
Unmethylated C's in Unknown context 12145715
Percentage methylation (CpG context) 12.1%
Percentage methylation (CHG context) 0.4%
Percentage methylation (CHH context) 0.4%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 22935222 original top strand
CTOT 23179245 complementary to original top strand
CTOB 22958193 complementary to original bottom strand
OB 22732926 original bottom strand

Deduplication

Alignments analysed 91805586
Unique alignments 83853098
Duplicates removed 7952488
Duplicated alignments were found at 6589375 different positions

Cytosine Methylation after Extraction

Total C's analysed 2298440911
Methylated C's in CpG context 34690212
Methylated C's in CHG context 1663625
Methylated C's in CHH context 6720771
Unmethylated C's in CpG context 254538680
Unmethylated C's in CHG context 397912346
Unmethylated C's in CHH context 1602915277
Percentage methylation (CpG context) 12.0%
Percentage methylation (CHG context) N/A%
Percentage methylation (CHH context) N/A%

M-Bias Plot