Bismark report for: /gscratch/srlab/sr320/data/cvirg/31M_R1_val_1.fq.gz and /gscratch/srlab/sr320/data/cvirg/31M_R2_val_2.fq.gz (version: v0.22.3) Bismark was run with Bowtie 2 against the bisulfite genome of /gscratch/srlab/sr320/data/Cvirg-genome/ with the specified options: -q --score-min L,0,-0.6 -p 4 --reorder --ignore-quals --no-mixed --no-discordant --dovetail --maxins 500 Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Final Alignment report ====================== Sequence pairs analysed in total: 141797876 Number of paired-end alignments with a unique best hit: 83818940 Mapping efficiency: 59.1% Sequence pairs with no alignments under any condition: 35155949 Sequence pairs did not map uniquely: 22822987 Sequence pairs which were discarded because genomic sequence could not be extracted: 62 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 20896957 ((converted) top strand) GA/CT/CT: 21003842 (complementary to (converted) top strand) GA/CT/GA: 21013523 (complementary to (converted) bottom strand) CT/GA/GA: 20904556 ((converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 3471808061 Total methylated C's in CpG context: 63220038 Total methylated C's in CHG context: 2486404 Total methylated C's in CHH context: 10975824 Total methylated C's in Unknown context: 286319 Total unmethylated C's in CpG context: 400980153 Total unmethylated C's in CHG context: 630300848 Total unmethylated C's in CHH context: 2363844794 Total unmethylated C's in Unknown context: 10200137 C methylated in CpG context: 13.6% C methylated in CHG context: 0.4% C methylated in CHH context: 0.5% C methylated in unknown context (CN or CHN): 2.7% Bismark completed in 0d 14h 17m 51s