Bismark

Bismark Processing Report

/gscratch/srlab/sr320/data/cvirg/31M_R1_val_1.fq.gz and /gscratch/srlab/sr320/data/cvirg/31M_R2_val_2.fq.gz

Data processed at 13:36:54 on 2021-08-12


Alignment Stats

Sequence pairs analysed in total 141797876
Paired-end alignments with a unique best hit 83818940
Pairs without alignments under any condition 35155949
Pairs that did not map uniquely 22822987
Genomic sequence context not extractable (edges of chromosomes) 62

Cytosine Methylation

Total C's analysed 3471808061
Methylated C's in CpG context 63220038
Methylated C's in CHG context 2486404
Methylated C's in CHH context 10975824
Methylated C's in Unknown context 286319
Unmethylated C's in CpG context 400980153
Unmethylated C's in CHG context 630300848
Unmethylated C's in CHH context 2363844794
Unmethylated C's in Unknown context 10200137
Percentage methylation (CpG context) 13.6%
Percentage methylation (CHG context) 0.4%
Percentage methylation (CHH context) 0.5%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 20896957 original top strand
CTOT 21003842 complementary to original top strand
CTOB 21013523 complementary to original bottom strand
OB 20904556 original bottom strand

Deduplication

Alignments analysed 83818878
Unique alignments 77191386
Duplicates removed 6627492
Duplicated alignments were found at 6055015 different positions

Cytosine Methylation after Extraction

Total C's analysed 1930776015
Methylated C's in CpG context 34183478
Methylated C's in CHG context 1349709
Methylated C's in CHH context 5922078
Unmethylated C's in CpG context 220646744
Unmethylated C's in CHG context 346999276
Unmethylated C's in CHH context 1321674730
Percentage methylation (CpG context) 13.4%
Percentage methylation (CHG context) N/A%
Percentage methylation (CHH context) N/A%

M-Bias Plot