Bismark report for: /gscratch/srlab/sr320/data/cvirg/29F_R1_val_1.fq.gz and /gscratch/srlab/sr320/data/cvirg/29F_R2_val_2.fq.gz (version: v0.22.3) Bismark was run with Bowtie 2 against the bisulfite genome of /gscratch/srlab/sr320/data/Cvirg-genome/ with the specified options: -q --score-min L,0,-0.6 -p 4 --reorder --ignore-quals --no-mixed --no-discordant --dovetail --maxins 500 Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Final Alignment report ====================== Sequence pairs analysed in total: 137641794 Number of paired-end alignments with a unique best hit: 78897899 Mapping efficiency: 57.3% Sequence pairs with no alignments under any condition: 39744516 Sequence pairs did not map uniquely: 18999379 Sequence pairs which were discarded because genomic sequence could not be extracted: 394 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 19767756 ((converted) top strand) GA/CT/CT: 19760348 (complementary to (converted) top strand) GA/CT/GA: 19683219 (complementary to (converted) bottom strand) CT/GA/GA: 19686182 ((converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 3335365003 Total methylated C's in CpG context: 49477481 Total methylated C's in CHG context: 2353635 Total methylated C's in CHH context: 9844711 Total methylated C's in Unknown context: 215328 Total unmethylated C's in CpG context: 373680681 Total unmethylated C's in CHG context: 582136274 Total unmethylated C's in CHH context: 2317872221 Total unmethylated C's in Unknown context: 10880597 C methylated in CpG context: 11.7% C methylated in CHG context: 0.4% C methylated in CHH context: 0.4% C methylated in unknown context (CN or CHN): 1.9% Bismark completed in 0d 13h 54m 32s