Bismark

Bismark Processing Report

/gscratch/srlab/sr320/data/cvirg/29F_R1_val_1.fq.gz and /gscratch/srlab/sr320/data/cvirg/29F_R2_val_2.fq.gz

Data processed at 13:36:54 on 2021-08-12


Alignment Stats

Sequence pairs analysed in total 137641794
Paired-end alignments with a unique best hit 78897899
Pairs without alignments under any condition 39744516
Pairs that did not map uniquely 18999379
Genomic sequence context not extractable (edges of chromosomes) 394

Cytosine Methylation

Total C's analysed 3335365003
Methylated C's in CpG context 49477481
Methylated C's in CHG context 2353635
Methylated C's in CHH context 9844711
Methylated C's in Unknown context 215328
Unmethylated C's in CpG context 373680681
Unmethylated C's in CHG context 582136274
Unmethylated C's in CHH context 2317872221
Unmethylated C's in Unknown context 10880597
Percentage methylation (CpG context) 11.7%
Percentage methylation (CHG context) 0.4%
Percentage methylation (CHH context) 0.4%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 19767756 original top strand
CTOT 19760348 complementary to original top strand
CTOB 19683219 complementary to original bottom strand
OB 19686182 original bottom strand

Deduplication

Alignments analysed 78897505
Unique alignments 72910041
Duplicates removed 5987464
Duplicated alignments were found at 5471094 different positions

Cytosine Methylation after Extraction

Total C's analysed 2061199527
Methylated C's in CpG context 29672752
Methylated C's in CHG context 1416706
Methylated C's in CHH context 5937298
Unmethylated C's in CpG context 228068733
Unmethylated C's in CHG context 355765857
Unmethylated C's in CHH context 1440338181
Percentage methylation (CpG context) 11.5%
Percentage methylation (CHG context) N/A%
Percentage methylation (CHH context) N/A%

M-Bias Plot