Bismark report for: /gscratch/srlab/sr320/data/cvirg/22F_R1_val_1.fq.gz and /gscratch/srlab/sr320/data/cvirg/22F_R2_val_2.fq.gz (version: v0.22.3) Bismark was run with Bowtie 2 against the bisulfite genome of /gscratch/srlab/sr320/data/Cvirg-genome/ with the specified options: -q --score-min L,0,-0.6 -p 4 --reorder --ignore-quals --no-mixed --no-discordant --dovetail --maxins 500 Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Final Alignment report ====================== Sequence pairs analysed in total: 125853047 Number of paired-end alignments with a unique best hit: 72295488 Mapping efficiency: 57.4% Sequence pairs with no alignments under any condition: 36996632 Sequence pairs did not map uniquely: 16560927 Sequence pairs which were discarded because genomic sequence could not be extracted: 763 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 18147434 ((converted) top strand) GA/CT/CT: 18110201 (complementary to (converted) top strand) GA/CT/GA: 17994896 (complementary to (converted) bottom strand) CT/GA/GA: 18042194 ((converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 3086134405 Total methylated C's in CpG context: 46627292 Total methylated C's in CHG context: 2254195 Total methylated C's in CHH context: 9294865 Total methylated C's in Unknown context: 202722 Total unmethylated C's in CpG context: 341506695 Total unmethylated C's in CHG context: 540076097 Total unmethylated C's in CHH context: 2146375261 Total unmethylated C's in Unknown context: 10114623 C methylated in CpG context: 12.0% C methylated in CHG context: 0.4% C methylated in CHH context: 0.4% C methylated in unknown context (CN or CHN): 2.0% Bismark completed in 0d 12h 40m 40s