Bismark

Bismark Processing Report

/gscratch/srlab/sr320/data/cvirg/22F_R1_val_1.fq.gz and /gscratch/srlab/sr320/data/cvirg/22F_R2_val_2.fq.gz

Data processed at 13:36:54 on 2021-08-12


Alignment Stats

Sequence pairs analysed in total 125853047
Paired-end alignments with a unique best hit 72295488
Pairs without alignments under any condition 36996632
Pairs that did not map uniquely 16560927
Genomic sequence context not extractable (edges of chromosomes) 763

Cytosine Methylation

Total C's analysed 3086134405
Methylated C's in CpG context 46627292
Methylated C's in CHG context 2254195
Methylated C's in CHH context 9294865
Methylated C's in Unknown context 202722
Unmethylated C's in CpG context 341506695
Unmethylated C's in CHG context 540076097
Unmethylated C's in CHH context 2146375261
Unmethylated C's in Unknown context 10114623
Percentage methylation (CpG context) 12.0%
Percentage methylation (CHG context) 0.4%
Percentage methylation (CHH context) 0.4%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 18147434 original top strand
CTOT 18110201 complementary to original top strand
CTOB 17994896 complementary to original bottom strand
OB 18042194 original bottom strand

Deduplication

Alignments analysed 72294725
Unique alignments 66746852
Duplicates removed 5547873
Duplicated alignments were found at 4871373 different positions

Cytosine Methylation after Extraction

Total C's analysed 1932255721
Methylated C's in CpG context 28515624
Methylated C's in CHG context 1366744
Methylated C's in CHH context 5635575
Unmethylated C's in CpG context 210931753
Unmethylated C's in CHG context 334520859
Unmethylated C's in CHH context 1351285166
Percentage methylation (CpG context) 11.9%
Percentage methylation (CHG context) N/A%
Percentage methylation (CHH context) N/A%

M-Bias Plot