Bismark report for: /gscratch/srlab/sr320/data/cvirg/16F_R1_val_1.fq.gz and /gscratch/srlab/sr320/data/cvirg/16F_R2_val_2.fq.gz (version: v0.22.3) Bismark was run with Bowtie 2 against the bisulfite genome of /gscratch/srlab/sr320/data/Cvirg-genome/ with the specified options: -q --score-min L,0,-0.6 -p 4 --reorder --ignore-quals --no-mixed --no-discordant --dovetail --maxins 500 Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Final Alignment report ====================== Sequence pairs analysed in total: 184170994 Number of paired-end alignments with a unique best hit: 103096144 Mapping efficiency: 56.0% Sequence pairs with no alignments under any condition: 53479707 Sequence pairs did not map uniquely: 27595143 Sequence pairs which were discarded because genomic sequence could not be extracted: 342 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 25858184 ((converted) top strand) GA/CT/CT: 25751911 (complementary to (converted) top strand) GA/CT/GA: 25694337 (complementary to (converted) bottom strand) CT/GA/GA: 25791370 ((converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 4112186084 Total methylated C's in CpG context: 67140496 Total methylated C's in CHG context: 3202091 Total methylated C's in CHH context: 13160640 Total methylated C's in Unknown context: 286211 Total unmethylated C's in CpG context: 470893717 Total unmethylated C's in CHG context: 734473042 Total unmethylated C's in CHH context: 2823316098 Total unmethylated C's in Unknown context: 13163858 C methylated in CpG context: 12.5% C methylated in CHG context: 0.4% C methylated in CHH context: 0.5% C methylated in unknown context (CN or CHN): 2.1% Bismark completed in 0d 17h 35m 50s