Bismark

Bismark Processing Report

/gscratch/srlab/sr320/data/cvirg/16F_R1_val_1.fq.gz and /gscratch/srlab/sr320/data/cvirg/16F_R2_val_2.fq.gz

Data processed at 13:36:53 on 2021-08-12


Alignment Stats

Sequence pairs analysed in total 184170994
Paired-end alignments with a unique best hit 103096144
Pairs without alignments under any condition 53479707
Pairs that did not map uniquely 27595143
Genomic sequence context not extractable (edges of chromosomes) 342

Cytosine Methylation

Total C's analysed 4112186084
Methylated C's in CpG context 67140496
Methylated C's in CHG context 3202091
Methylated C's in CHH context 13160640
Methylated C's in Unknown context 286211
Unmethylated C's in CpG context 470893717
Unmethylated C's in CHG context 734473042
Unmethylated C's in CHH context 2823316098
Unmethylated C's in Unknown context 13163858
Percentage methylation (CpG context) 12.5%
Percentage methylation (CHG context) 0.4%
Percentage methylation (CHH context) 0.5%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 25858184 original top strand
CTOT 25751911 complementary to original top strand
CTOB 25694337 complementary to original bottom strand
OB 25791370 original bottom strand

Deduplication

Alignments analysed 103095802
Unique alignments 94179724
Duplicates removed 8916078
Duplicated alignments were found at 8089051 different positions

Cytosine Methylation after Extraction

Total C's analysed 2377770409
Methylated C's in CpG context 37346078
Methylated C's in CHG context 1784502
Methylated C's in CHH context 7425685
Unmethylated C's in CpG context 268205283
Unmethylated C's in CHG context 418913612
Unmethylated C's in CHH context 1644095249
Percentage methylation (CpG context) 12.2%
Percentage methylation (CHG context) N/A%
Percentage methylation (CHH context) N/A%

M-Bias Plot