Bismark report for: /gscratch/srlab/sr320/data/cvirg/13M_R1_val_1.fq.gz and /gscratch/srlab/sr320/data/cvirg/13M_R2_val_2.fq.gz (version: v0.22.3) Bismark was run with Bowtie 2 against the bisulfite genome of /gscratch/srlab/sr320/data/Cvirg-genome/ with the specified options: -q --score-min L,0,-0.6 -p 4 --reorder --ignore-quals --no-mixed --no-discordant --dovetail --maxins 500 Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Final Alignment report ====================== Sequence pairs analysed in total: 217295607 Number of paired-end alignments with a unique best hit: 124898218 Mapping efficiency: 57.5% Sequence pairs with no alignments under any condition: 57467614 Sequence pairs did not map uniquely: 34929775 Sequence pairs which were discarded because genomic sequence could not be extracted: 132 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 31254632 ((converted) top strand) GA/CT/CT: 31176521 (complementary to (converted) top strand) GA/CT/GA: 31189603 (complementary to (converted) bottom strand) CT/GA/GA: 31277330 ((converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 5245651403 Total methylated C's in CpG context: 92402811 Total methylated C's in CHG context: 3795552 Total methylated C's in CHH context: 15373578 Total methylated C's in Unknown context: 422443 Total unmethylated C's in CpG context: 616600861 Total unmethylated C's in CHG context: 959301177 Total unmethylated C's in CHH context: 3558177424 Total unmethylated C's in Unknown context: 16442872 C methylated in CpG context: 13.0% C methylated in CHG context: 0.4% C methylated in CHH context: 0.4% C methylated in unknown context (CN or CHN): 2.5% Bismark completed in 0d 21h 30m 9s