Bismark

Bismark Processing Report

/gscratch/srlab/sr320/data/cvirg/13M_R1_val_1.fq.gz and /gscratch/srlab/sr320/data/cvirg/13M_R2_val_2.fq.gz

Data processed at 13:36:53 on 2021-08-12


Alignment Stats

Sequence pairs analysed in total 217295607
Paired-end alignments with a unique best hit 124898218
Pairs without alignments under any condition 57467614
Pairs that did not map uniquely 34929775
Genomic sequence context not extractable (edges of chromosomes) 132

Cytosine Methylation

Total C's analysed 5245651403
Methylated C's in CpG context 92402811
Methylated C's in CHG context 3795552
Methylated C's in CHH context 15373578
Methylated C's in Unknown context 422443
Unmethylated C's in CpG context 616600861
Unmethylated C's in CHG context 959301177
Unmethylated C's in CHH context 3558177424
Unmethylated C's in Unknown context 16442872
Percentage methylation (CpG context) 13.0%
Percentage methylation (CHG context) 0.4%
Percentage methylation (CHH context) 0.4%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 31254632 original top strand
CTOT 31176521 complementary to original top strand
CTOB 31189603 complementary to original bottom strand
OB 31277330 original bottom strand

Deduplication

Alignments analysed 124898086
Unique alignments 112840017
Duplicates removed 12058069
Duplicated alignments were found at 10786346 different positions

Cytosine Methylation after Extraction

Total C's analysed 2895601370
Methylated C's in CpG context 49622161
Methylated C's in CHG context 2041725
Methylated C's in CHH context 8365379
Unmethylated C's in CpG context 336438857
Unmethylated C's in CHG context 523821535
Unmethylated C's in CHH context 1975311713
Percentage methylation (CpG context) 12.9%
Percentage methylation (CHG context) N/A%
Percentage methylation (CHH context) N/A%

M-Bias Plot