Bismark

Bismark Processing Report

/gscratch/srlab/sr320/data/cvirg/12M_R1_val_1.fq.gz and /gscratch/srlab/sr320/data/cvirg/12M_R2_val_2.fq.gz

Data processed at 13:36:53 on 2021-08-12


Alignment Stats

Sequence pairs analysed in total 192759606
Paired-end alignments with a unique best hit 112355995
Pairs without alignments under any condition 51523832
Pairs that did not map uniquely 28879779
Genomic sequence context not extractable (edges of chromosomes) 106

Cytosine Methylation

Total C's analysed 4754445853
Methylated C's in CpG context 84765766
Methylated C's in CHG context 3297706
Methylated C's in CHH context 13082502
Methylated C's in Unknown context 347166
Unmethylated C's in CpG context 534551994
Unmethylated C's in CHG context 849789941
Unmethylated C's in CHH context 3268957944
Unmethylated C's in Unknown context 15161189
Percentage methylation (CpG context) 13.7%
Percentage methylation (CHG context) 0.4%
Percentage methylation (CHH context) 0.4%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 28265285 original top strand
CTOT 27896844 complementary to original top strand
CTOB 27910481 complementary to original bottom strand
OB 28283279 original bottom strand

Deduplication

Alignments analysed 112355889
Unique alignments 101143882
Duplicates removed 11212007
Duplicated alignments were found at 10064165 different positions

Cytosine Methylation after Extraction

Total C's analysed 2725400659
Methylated C's in CpG context 47153262
Methylated C's in CHG context 1834477
Methylated C's in CHH context 7382974
Unmethylated C's in CpG context 302909324
Unmethylated C's in CHG context 481680820
Unmethylated C's in CHH context 1884439802
Percentage methylation (CpG context) 13.5%
Percentage methylation (CHG context) N/A%
Percentage methylation (CHH context) N/A%

M-Bias Plot