Bismark

Bismark Processing Report

/gscratch/srlab/sr320/data/olurida-bs/decomp//zr1394_all_s456_trimmed.fq

Data processed at 00:07 on 2022-07-05


Alignment Stats

Sequences analysed in total 605809374
Single-end alignments with a unique best hit 184983541
Sequences without alignments under any condition 179447426
Sequences that did not map uniquely 241378407
Genomic sequence context not extractable (edges of chromosomes) 3

Cytosine Methylation

Total C's analysed 1150617798
Methylated C's in CpG context 174688062
Methylated C's in CHG context 5203372
Methylated C's in CHH context 61461946
Methylated C's in Unknown context 52708
Unmethylated C's in CpG context 30920274
Unmethylated C's in CHG context 230451229
Unmethylated C's in CHH context 647892915
Unmethylated C's in Unknown context 362895
Percentage methylation (CpG context) 85.0%
Percentage methylation (CHG context) 2.2%
Percentage methylation (CHH context) 8.7%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 42432699 original top strand
CTOT 50418251 complementary to original top strand
CTOB 50016394 complementary to original bottom strand
OB 42116194 original bottom strand

Deduplication

Alignments analysed 184983538
Unique alignments 60848155
Duplicates removed 124135383
Duplicated alignments were found at 20776616 different positions

Cytosine Methylation after Extraction

Total C's analysed 362887673
Methylated C's in CpG context 37503474
Methylated C's in CHG context 2098223
Methylated C's in CHH context 31599525
Unmethylated C's in CpG context 11075480
Unmethylated C's in CHG context 61396504
Unmethylated C's in CHH context 219214467
Percentage methylation (CpG context) 77.2%
Percentage methylation (CHG context) 3.3%
Percentage methylation (CHH context) 12.6%

M-Bias Plot