Bismark

Bismark Processing Report

/gscratch/srlab/sr320/data/olurida-bs/decomp//zr1394_9_s456_trimmed.fq

Data processed at 00:07 on 2022-07-05


Alignment Stats

Sequences analysed in total 77350922
Single-end alignments with a unique best hit 25240310
Sequences without alignments under any condition 22010659
Sequences that did not map uniquely 30099953
Genomic sequence context not extractable (edges of chromosomes) 1

Cytosine Methylation

Total C's analysed 150063744
Methylated C's in CpG context 16646053
Methylated C's in CHG context 573090
Methylated C's in CHH context 8128400
Methylated C's in Unknown context 5241
Unmethylated C's in CpG context 4057189
Unmethylated C's in CHG context 28845101
Unmethylated C's in CHH context 91813911
Unmethylated C's in Unknown context 42803
Percentage methylation (CpG context) 80.4%
Percentage methylation (CHG context) 1.9%
Percentage methylation (CHH context) 8.1%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 5674291 original top strand
CTOT 6980377 complementary to original top strand
CTOB 6938554 complementary to original bottom strand
OB 5647087 original bottom strand

Deduplication

Alignments analysed 25240309
Unique alignments 16557255
Duplicates removed 8683054
Duplicated alignments were found at 3224875 different positions

Cytosine Methylation after Extraction

Total C's analysed 96977575
Methylated C's in CpG context 9515451
Methylated C's in CHG context 403873
Methylated C's in CHH context 6284505
Unmethylated C's in CpG context 2696345
Unmethylated C's in CHG context 17686365
Unmethylated C's in CHH context 60391036
Percentage methylation (CpG context) 77.9%
Percentage methylation (CHG context) 2.2%
Percentage methylation (CHH context) 9.4%

M-Bias Plot