Bismark

Bismark Processing Report

/gscratch/srlab/sr320/data/olurida-bs/decomp//zr1394_8_s456_trimmed.fq

Data processed at 00:07 on 2022-07-05


Alignment Stats

Sequences analysed in total 38697189
Single-end alignments with a unique best hit 12035925
Sequences without alignments under any condition 11580098
Sequences that did not map uniquely 15081166
Genomic sequence context not extractable (edges of chromosomes) 0

Cytosine Methylation

Total C's analysed 73932964
Methylated C's in CpG context 10514623
Methylated C's in CHG context 336033
Methylated C's in CHH context 4067854
Methylated C's in Unknown context 3281
Unmethylated C's in CpG context 1610852
Unmethylated C's in CHG context 14707547
Unmethylated C's in CHH context 42696055
Unmethylated C's in Unknown context 21955
Percentage methylation (CpG context) 86.7%
Percentage methylation (CHG context) 2.2%
Percentage methylation (CHH context) 8.7%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 2668893 original top strand
CTOT 3365800 complementary to original top strand
CTOB 3345855 complementary to original bottom strand
OB 2655377 original bottom strand

Deduplication

Alignments analysed 12035925
Unique alignments 8480861
Duplicates removed 3555064
Duplicated alignments were found at 1451198 different positions

Cytosine Methylation after Extraction

Total C's analysed 51318369
Methylated C's in CpG context 6600233
Methylated C's in CHG context 246352
Methylated C's in CHH context 3250952
Unmethylated C's in CpG context 1127010
Unmethylated C's in CHG context 9781878
Unmethylated C's in CHH context 30311944
Percentage methylation (CpG context) 85.4%
Percentage methylation (CHG context) 2.5%
Percentage methylation (CHH context) 9.7%

M-Bias Plot