Bismark

Bismark Processing Report

/gscratch/srlab/sr320/data/olurida-bs/decomp//zr1394_7_s456_trimmed.fq

Data processed at 00:07 on 2022-07-05


Alignment Stats

Sequences analysed in total 49730113
Single-end alignments with a unique best hit 15378146
Sequences without alignments under any condition 15119845
Sequences that did not map uniquely 19232122
Genomic sequence context not extractable (edges of chromosomes) 0

Cytosine Methylation

Total C's analysed 94177787
Methylated C's in CpG context 12731776
Methylated C's in CHG context 439419
Methylated C's in CHH context 5211666
Methylated C's in Unknown context 4444
Unmethylated C's in CpG context 2510434
Unmethylated C's in CHG context 18674935
Unmethylated C's in CHH context 54609557
Unmethylated C's in Unknown context 29264
Percentage methylation (CpG context) 83.5%
Percentage methylation (CHG context) 2.3%
Percentage methylation (CHH context) 8.7%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 3512207 original top strand
CTOT 4205558 complementary to original top strand
CTOB 4169989 complementary to original bottom strand
OB 3490392 original bottom strand

Deduplication

Alignments analysed 15378146
Unique alignments 10691236
Duplicates removed 4686910
Duplicated alignments were found at 1882201 different positions

Cytosine Methylation after Extraction

Total C's analysed 64455591
Methylated C's in CpG context 7807245
Methylated C's in CHG context 318516
Methylated C's in CHH context 4157485
Unmethylated C's in CpG context 1756856
Unmethylated C's in CHG context 12199183
Unmethylated C's in CHH context 38216306
Percentage methylation (CpG context) 81.6%
Percentage methylation (CHG context) 2.5%
Percentage methylation (CHH context) 9.8%

M-Bias Plot