Bismark

Bismark Processing Report

/gscratch/srlab/sr320/data/olurida-bs/decomp//zr1394_6_s456_trimmed.fq

Data processed at 00:07 on 2022-07-05


Alignment Stats

Sequences analysed in total 46455148
Single-end alignments with a unique best hit 15528129
Sequences without alignments under any condition 14024669
Sequences that did not map uniquely 16902350
Genomic sequence context not extractable (edges of chromosomes) 0

Cytosine Methylation

Total C's analysed 90762894
Methylated C's in CpG context 9013613
Methylated C's in CHG context 404127
Methylated C's in CHH context 5399151
Methylated C's in Unknown context 3111
Unmethylated C's in CpG context 2337404
Unmethylated C's in CHG context 17531974
Unmethylated C's in CHH context 56076625
Unmethylated C's in Unknown context 24469
Percentage methylation (CpG context) 79.4%
Percentage methylation (CHG context) 2.3%
Percentage methylation (CHH context) 8.8%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 3458972 original top strand
CTOT 4328400 complementary to original top strand
CTOB 4297380 complementary to original bottom strand
OB 3443377 original bottom strand

Deduplication

Alignments analysed 15528129
Unique alignments 11813169
Duplicates removed 3714960
Duplicated alignments were found at 1783653 different positions

Cytosine Methylation after Extraction

Total C's analysed 68369911
Methylated C's in CpG context 6268748
Methylated C's in CHG context 306276
Methylated C's in CHH context 4421749
Unmethylated C's in CpG context 1806827
Unmethylated C's in CHG context 12801677
Unmethylated C's in CHH context 42764634
Percentage methylation (CpG context) 77.6%
Percentage methylation (CHG context) 2.3%
Percentage methylation (CHH context) 9.4%

M-Bias Plot