Bismark

Bismark Processing Report

/gscratch/srlab/sr320/data/olurida-bs/decomp//zr1394_5_s456_trimmed.fq

Data processed at 00:07 on 2022-07-05


Alignment Stats

Sequences analysed in total 47401736
Single-end alignments with a unique best hit 15747029
Sequences without alignments under any condition 14134741
Sequences that did not map uniquely 17519966
Genomic sequence context not extractable (edges of chromosomes) 0

Cytosine Methylation

Total C's analysed 93113950
Methylated C's in CpG context 9933157
Methylated C's in CHG context 400913
Methylated C's in CHH context 5646633
Methylated C's in Unknown context 3851
Unmethylated C's in CpG context 3086035
Unmethylated C's in CHG context 18513739
Unmethylated C's in CHH context 55533473
Unmethylated C's in Unknown context 25960
Percentage methylation (CpG context) 76.3%
Percentage methylation (CHG context) 2.1%
Percentage methylation (CHH context) 9.2%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 3575045 original top strand
CTOT 4329383 complementary to original top strand
CTOB 4286298 complementary to original bottom strand
OB 3556303 original bottom strand

Deduplication

Alignments analysed 15747029
Unique alignments 11776287
Duplicates removed 3970742
Duplicated alignments were found at 1836778 different positions

Cytosine Methylation after Extraction

Total C's analysed 68921607
Methylated C's in CpG context 6669103
Methylated C's in CHG context 306607
Methylated C's in CHH context 4610637
Unmethylated C's in CpG context 2370609
Unmethylated C's in CHG context 13169917
Unmethylated C's in CHH context 41794734
Percentage methylation (CpG context) 73.8%
Percentage methylation (CHG context) 2.3%
Percentage methylation (CHH context) 9.9%

M-Bias Plot