Bismark report for: /gscratch/srlab/sr320/data/olurida-bs/decomp//zr1394_4_s456_trimmed.fq (version: v0.21.0) Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Bismark was run with Bowtie 2 against the bisulfite genome of /gscratch/srlab/sr320/data/olurida-bs/v98/ with the specified options: -q --score-min L,0,-0.2 -p 4 --reorder --ignore-quals Final Alignment report ====================== Sequences analysed in total: 44905078 Number of alignments with a unique best hit from the different alignments: 13872301 Mapping efficiency: 30.9% Sequences with no alignments under any condition: 13715942 Sequences did not map uniquely: 17316835 Sequences which were discarded because genomic sequence could not be extracted: 0 Number of sequences with unique best (first) alignment came from the bowtie output: CT/CT: 3093739 ((converted) top strand) CT/GA: 3070130 ((converted) bottom strand) GA/CT: 3864472 (complementary to (converted) top strand) GA/GA: 3843960 (complementary to (converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 85006558 Total methylated C's in CpG context: 11729361 Total methylated C's in CHG context: 405370 Total methylated C's in CHH context: 4793316 Total methylated C's in Unknown context: 3754 Total unmethylated C's in CpG context: 1940399 Total unmethylated C's in CHG context: 16871129 Total unmethylated C's in CHH context: 49266983 Total unmethylated C's in Unknown context: 25310 C methylated in CpG context: 85.8% C methylated in CHG context: 2.3% C methylated in CHH context: 8.9% C methylated in Unknown context (CN or CHN): 12.9% Bismark completed in 0d 0h 59m 56s