Bismark

Bismark Processing Report

/gscratch/srlab/sr320/data/olurida-bs/decomp//zr1394_4_s456_trimmed.fq

Data processed at 00:07 on 2022-07-05


Alignment Stats

Sequences analysed in total 44905078
Single-end alignments with a unique best hit 13872301
Sequences without alignments under any condition 13715942
Sequences that did not map uniquely 17316835
Genomic sequence context not extractable (edges of chromosomes) 0

Cytosine Methylation

Total C's analysed 85006558
Methylated C's in CpG context 11729361
Methylated C's in CHG context 405370
Methylated C's in CHH context 4793316
Methylated C's in Unknown context 3754
Unmethylated C's in CpG context 1940399
Unmethylated C's in CHG context 16871129
Unmethylated C's in CHH context 49266983
Unmethylated C's in Unknown context 25310
Percentage methylation (CpG context) 85.8%
Percentage methylation (CHG context) 2.3%
Percentage methylation (CHH context) 8.9%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 3093739 original top strand
CTOT 3864472 complementary to original top strand
CTOB 3843960 complementary to original bottom strand
OB 3070130 original bottom strand

Deduplication

Alignments analysed 13872301
Unique alignments 9514726
Duplicates removed 4357575
Duplicated alignments were found at 1707009 different positions

Cytosine Methylation after Extraction

Total C's analysed 57380051
Methylated C's in CpG context 7093417
Methylated C's in CHG context 290465
Methylated C's in CHH context 3757271
Unmethylated C's in CpG context 1317260
Unmethylated C's in CHG context 10875988
Unmethylated C's in CHH context 34045650
Percentage methylation (CpG context) 84.3%
Percentage methylation (CHG context) 2.6%
Percentage methylation (CHH context) 9.9%

M-Bias Plot