Bismark

Bismark Processing Report

/gscratch/srlab/sr320/data/olurida-bs/decomp//zr1394_2_s456_trimmed.fq

Data processed at 00:07 on 2022-07-05


Alignment Stats

Sequences analysed in total 46105140
Single-end alignments with a unique best hit 14822810
Sequences without alignments under any condition 14100920
Sequences that did not map uniquely 17181410
Genomic sequence context not extractable (edges of chromosomes) 0

Cytosine Methylation

Total C's analysed 89520561
Methylated C's in CpG context 11404907
Methylated C's in CHG context 408791
Methylated C's in CHH context 5222368
Methylated C's in Unknown context 3817
Unmethylated C's in CpG context 2330389
Unmethylated C's in CHG context 17926332
Unmethylated C's in CHH context 52227774
Unmethylated C's in Unknown context 25383
Percentage methylation (CpG context) 83.0%
Percentage methylation (CHG context) 2.2%
Percentage methylation (CHH context) 9.1%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 3308195 original top strand
CTOT 4126694 complementary to original top strand
CTOB 4098178 complementary to original bottom strand
OB 3289743 original bottom strand

Deduplication

Alignments analysed 14822810
Unique alignments 10272618
Duplicates removed 4550192
Duplicated alignments were found at 1967952 different positions

Cytosine Methylation after Extraction

Total C's analysed 61305714
Methylated C's in CpG context 7074055
Methylated C's in CHG context 296036
Methylated C's in CHH context 4093092
Unmethylated C's in CpG context 1645137
Unmethylated C's in CHG context 11724386
Unmethylated C's in CHH context 36473008
Percentage methylation (CpG context) 81.1%
Percentage methylation (CHG context) 2.5%
Percentage methylation (CHH context) 10.1%

M-Bias Plot