Bismark

Bismark Processing Report

/gscratch/srlab/sr320/data/olurida-bs/decomp//zr1394_1_s456_trimmed.fq

Data processed at 00:07 on 2022-07-05


Alignment Stats

Sequences analysed in total 46792286
Single-end alignments with a unique best hit 14534760
Sequences without alignments under any condition 14155170
Sequences that did not map uniquely 18102356
Genomic sequence context not extractable (edges of chromosomes) 1

Cytosine Methylation

Total C's analysed 88484060
Methylated C's in CpG context 11703467
Methylated C's in CHG context 417556
Methylated C's in CHH context 5009940
Methylated C's in Unknown context 3647
Unmethylated C's in CpG context 2470410
Unmethylated C's in CHG context 17414271
Unmethylated C's in CHH context 51468416
Unmethylated C's in Unknown context 25966
Percentage methylation (CpG context) 82.6%
Percentage methylation (CHG context) 2.3%
Percentage methylation (CHH context) 8.9%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 3291229 original top strand
CTOT 4005984 complementary to original top strand
CTOB 3970736 complementary to original bottom strand
OB 3266810 original bottom strand

Deduplication

Alignments analysed 14534759
Unique alignments 10195324
Duplicates removed 4339435
Duplicated alignments were found at 1752249 different positions

Cytosine Methylation after Extraction

Total C's analysed 61108365
Methylated C's in CpG context 7206035
Methylated C's in CHG context 301119
Methylated C's in CHH context 3975802
Unmethylated C's in CpG context 1781863
Unmethylated C's in CHG context 11485875
Unmethylated C's in CHH context 36357671
Percentage methylation (CpG context) 80.2%
Percentage methylation (CHG context) 2.6%
Percentage methylation (CHH context) 9.9%

M-Bias Plot