Bismark report for: /gscratch/srlab/sr320/data/olurida-bs/decomp//zr1394_18_s456_trimmed.fq (version: v0.21.0) Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Bismark was run with Bowtie 2 against the bisulfite genome of /gscratch/srlab/sr320/data/olurida-bs/v98/ with the specified options: -q --score-min L,0,-0.2 -p 4 --reorder --ignore-quals Final Alignment report ====================== Sequences analysed in total: 55048106 Number of alignments with a unique best hit from the different alignments: 18068736 Mapping efficiency: 32.8% Sequences with no alignments under any condition: 15701339 Sequences did not map uniquely: 21278031 Sequences which were discarded because genomic sequence could not be extracted: 0 Number of sequences with unique best (first) alignment came from the bowtie output: CT/CT: 3964570 ((converted) top strand) CT/GA: 3939560 ((converted) bottom strand) GA/CT: 5099608 (complementary to (converted) top strand) GA/GA: 5064998 (complementary to (converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 109172180 Total methylated C's in CpG context: 13950028 Total methylated C's in CHG context: 447236 Total methylated C's in CHH context: 5912338 Total methylated C's in Unknown context: 4134 Total unmethylated C's in CpG context: 2826959 Total unmethylated C's in CHG context: 22196207 Total unmethylated C's in CHH context: 63839412 Total unmethylated C's in Unknown context: 30681 C methylated in CpG context: 83.1% C methylated in CHG context: 2.0% C methylated in CHH context: 8.5% C methylated in Unknown context (CN or CHN): 11.9% Bismark completed in 0d 1h 12m 53s