Bismark

Bismark Processing Report

/gscratch/srlab/sr320/data/olurida-bs/decomp//zr1394_18_s456_trimmed.fq

Data processed at 00:07 on 2022-07-05


Alignment Stats

Sequences analysed in total 55048106
Single-end alignments with a unique best hit 18068736
Sequences without alignments under any condition 15701339
Sequences that did not map uniquely 21278031
Genomic sequence context not extractable (edges of chromosomes) 0

Cytosine Methylation

Total C's analysed 109172180
Methylated C's in CpG context 13950028
Methylated C's in CHG context 447236
Methylated C's in CHH context 5912338
Methylated C's in Unknown context 4134
Unmethylated C's in CpG context 2826959
Unmethylated C's in CHG context 22196207
Unmethylated C's in CHH context 63839412
Unmethylated C's in Unknown context 30681
Percentage methylation (CpG context) 83.1%
Percentage methylation (CHG context) 2.0%
Percentage methylation (CHH context) 8.5%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 3964570 original top strand
CTOT 5099608 complementary to original top strand
CTOB 5064998 complementary to original bottom strand
OB 3939560 original bottom strand

Deduplication

Alignments analysed 18068736
Unique alignments 12017843
Duplicates removed 6050893
Duplicated alignments were found at 2424460 different positions

Cytosine Methylation after Extraction

Total C's analysed 71613889
Methylated C's in CpG context 8130684
Methylated C's in CHG context 313418
Methylated C's in CHH context 4538975
Unmethylated C's in CpG context 1878504
Unmethylated C's in CHG context 13868882
Unmethylated C's in CHH context 42883426
Percentage methylation (CpG context) 81.2%
Percentage methylation (CHG context) 2.2%
Percentage methylation (CHH context) 9.6%

M-Bias Plot