Bismark

Bismark Processing Report

/gscratch/srlab/sr320/data/olurida-bs/decomp//zr1394_17_s456_trimmed.fq

Data processed at 00:07 on 2022-07-05


Alignment Stats

Sequences analysed in total 42070546
Single-end alignments with a unique best hit 12831686
Sequences without alignments under any condition 12357075
Sequences that did not map uniquely 16881785
Genomic sequence context not extractable (edges of chromosomes) 0

Cytosine Methylation

Total C's analysed 79974402
Methylated C's in CpG context 12811467
Methylated C's in CHG context 384407
Methylated C's in CHH context 4482631
Methylated C's in Unknown context 3769
Unmethylated C's in CpG context 2034144
Unmethylated C's in CHG context 16655326
Unmethylated C's in CHH context 43606427
Unmethylated C's in Unknown context 25194
Percentage methylation (CpG context) 86.3%
Percentage methylation (CHG context) 2.3%
Percentage methylation (CHH context) 9.3%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 2921720 original top strand
CTOT 3516819 complementary to original top strand
CTOB 3492129 complementary to original bottom strand
OB 2901018 original bottom strand

Deduplication

Alignments analysed 12831686
Unique alignments 7796529
Duplicates removed 5035157
Duplicated alignments were found at 1717392 different positions

Cytosine Methylation after Extraction

Total C's analysed 47781647
Methylated C's in CpG context 6612578
Methylated C's in CHG context 249661
Methylated C's in CHH context 3301612
Unmethylated C's in CpG context 1191663
Unmethylated C's in CHG context 9371177
Unmethylated C's in CHH context 27054956
Percentage methylation (CpG context) 84.7%
Percentage methylation (CHG context) 2.6%
Percentage methylation (CHH context) 10.9%

M-Bias Plot