Bismark

Bismark Processing Report

/gscratch/srlab/sr320/data/olurida-bs/decomp//zr1394_16_s456_trimmed.fq

Data processed at 00:07 on 2022-07-05


Alignment Stats

Sequences analysed in total 74531056
Single-end alignments with a unique best hit 21286993
Sequences without alignments under any condition 22899250
Sequences that did not map uniquely 30344813
Genomic sequence context not extractable (edges of chromosomes) 1

Cytosine Methylation

Total C's analysed 133884872
Methylated C's in CpG context 22055874
Methylated C's in CHG context 631312
Methylated C's in CHH context 7117972
Methylated C's in Unknown context 6682
Unmethylated C's in CpG context 3714505
Unmethylated C's in CHG context 25955078
Unmethylated C's in CHH context 74410131
Unmethylated C's in Unknown context 43671
Percentage methylation (CpG context) 85.6%
Percentage methylation (CHG context) 2.4%
Percentage methylation (CHH context) 8.7%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 4885328 original top strand
CTOT 5798212 complementary to original top strand
CTOB 5752627 complementary to original bottom strand
OB 4850825 original bottom strand

Deduplication

Alignments analysed 21286992
Unique alignments 11846029
Duplicates removed 9440963
Duplicated alignments were found at 2727031 different positions

Cytosine Methylation after Extraction

Total C's analysed 72753637
Methylated C's in CpG context 9949262
Methylated C's in CHG context 384933
Methylated C's in CHH context 5094149
Unmethylated C's in CpG context 2085838
Unmethylated C's in CHG context 13053209
Unmethylated C's in CHH context 42186246
Percentage methylation (CpG context) 82.7%
Percentage methylation (CHG context) 2.9%
Percentage methylation (CHH context) 10.8%

M-Bias Plot