Alignment Stats
| Sequences analysed in total |
74531056 |
| Single-end alignments with a unique best hit |
21286993 |
| Sequences without alignments under any condition |
22899250 |
| Sequences that did not map uniquely |
30344813 |
| Genomic sequence context not extractable (edges of chromosomes) |
1 |
|
|
Cytosine Methylation
| Total C's analysed |
133884872 |
| Methylated C's in CpG context |
22055874 |
| Methylated C's in CHG context |
631312 |
| Methylated C's in CHH context |
7117972 |
| Methylated C's in Unknown context |
6682 |
| Unmethylated C's in CpG context |
3714505 |
| Unmethylated C's in CHG context |
25955078 |
| Unmethylated C's in CHH context |
74410131 |
| Unmethylated C's in Unknown context |
43671 |
| Percentage methylation (CpG context) |
85.6% |
| Percentage methylation (CHG context) |
2.4% |
| Percentage methylation (CHH context) |
8.7% |
| Methylated C's in Unknown context |
N/A% |
|
|
Alignment to Individual Bisulfite Strands
| OT |
4885328 |
original top strand |
| CTOT |
5798212 |
complementary to original top strand |
| CTOB |
5752627 |
complementary to original bottom strand |
| OB |
4850825 |
original bottom strand |
|
|
Deduplication
| Alignments analysed |
21286992 |
| Unique alignments |
11846029 |
| Duplicates removed |
9440963 |
| Duplicated alignments were found at 2727031 different positions |
|
|
Cytosine Methylation after Extraction
| Total C's analysed |
72753637 |
| Methylated C's in CpG context |
9949262 |
| Methylated C's in CHG context |
384933 |
| Methylated C's in CHH context |
5094149 |
| Unmethylated C's in CpG context |
2085838 |
| Unmethylated C's in CHG context |
13053209 |
| Unmethylated C's in CHH context |
42186246 |
| Percentage methylation (CpG context) |
82.7% |
| Percentage methylation (CHG context) |
2.9% |
| Percentage methylation (CHH context) |
10.8% |
|
|
M-Bias Plot