Bismark

Bismark Processing Report

/gscratch/srlab/sr320/data/olurida-bs/decomp//zr1394_15_s456_trimmed.fq

Data processed at 00:07 on 2022-07-05


Alignment Stats

Sequences analysed in total 67984205
Single-end alignments with a unique best hit 19910397
Sequences without alignments under any condition 20663417
Sequences that did not map uniquely 27410391
Genomic sequence context not extractable (edges of chromosomes) 0

Cytosine Methylation

Total C's analysed 126130939
Methylated C's in CpG context 21339729
Methylated C's in CHG context 602762
Methylated C's in CHH context 6674685
Methylated C's in Unknown context 6246
Unmethylated C's in CpG context 3060010
Unmethylated C's in CHG context 25313525
Unmethylated C's in CHH context 69140228
Unmethylated C's in Unknown context 42456
Percentage methylation (CpG context) 87.5%
Percentage methylation (CHG context) 2.3%
Percentage methylation (CHH context) 8.8%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 4492226 original top strand
CTOT 5501159 complementary to original top strand
CTOB 5454229 complementary to original bottom strand
OB 4462783 original bottom strand

Deduplication

Alignments analysed 19910397
Unique alignments 10728788
Duplicates removed 9181609
Duplicated alignments were found at 2576549 different positions

Cytosine Methylation after Extraction

Total C's analysed 66291550
Methylated C's in CpG context 9369352
Methylated C's in CHG context 360980
Methylated C's in CHH context 4662164
Unmethylated C's in CpG context 1667423
Unmethylated C's in CHG context 12247476
Unmethylated C's in CHH context 37984155
Percentage methylation (CpG context) 84.9%
Percentage methylation (CHG context) 2.9%
Percentage methylation (CHH context) 10.9%

M-Bias Plot